M.A. Thesis:

Comparison of two methods of histological test and pcr in detecting fraud in minced meat in Sabzevar city.

Abstract: Due to the economic value of meat, there is a possibility of using illegal animal tissues in meat products. In this study, the presence of unauthorized tissues in 30 minced meat samples in Sabzevar city has been investigated. This study was performed using two methods of histological test and PCR. The results of histological test show that in 30% of the samples, epithelial tissues (10%) and inflammation (20%) were observed. Also, the presence of fat was observed in three samples, which may have been caused by cross-sectional cutting. If you want meat programs by default, the presence of 10% fat and 10% connective tissue in minced meat is not an obstacle. In 10% of samples (3 samples) the percentage of unauthorized tissues (skin, meat and chicken bones) is 20% and in 20% of samples (6 samples) the percentage of unauthorized tissues is 20%. Therefore, in 30% of the samples, the standard is not observed and the minced meat is not of good quality. PCR test examined the presence of mutton and chicken tissue in minced meat. Based on the results, it has been shown that all samples contain mutton. Also, the results of PCR test showed the presence of unauthorized chicken tissues in nine of the thirty samples of the fraud study.

Keywords: Histological test, PCR test, cheating, minced meat.