M.A. Thesis:

Survey of VNN۱ expression in peoples with diabetes

Background: Type 2 diabetes is one of the most common metabolic disorders in the world, which is often unmarked in the early stages and can not be detected for several years. So early diagnosis of the disease will be effective in managing and treating it. As a result, continuous research is needed to identify specific biological markers. Different biomarkers have been identified to diagnose diabetes, in which case the VNN1 gene is also associated with diabetes. Also, the association of VNN1 gene in various diseases, including hypertension and cardiovascular disease, has been identified. In the present study, VNN1 activity was evaluated in blood samples of healthy individuals, fasting people and diabetic subjects. Materials and Methods: RNA was extracted from blood samples of diabetics, healthy people and fasting individuals, and its quantity and quality were determined using nanodrop and gel electrophoresis, and then cDNA synthesis was performed. Conditions were evaluated by optimum PCR technique and the target band after electrophoresis with duct gel and finally the gene expression was evaluated using real time PCR technique. Results: The results of this study showed that the level of VNN1 expression in blood samples of diabetic patients was 7.13 times higher than that of healthy subjects, and decreased in immature healthy subjects (0.29). Therefore, with further research, this gene can be used for diagnostic purposes and targeted therapies to help these patients.

Keywords: Type 2 Diabetes, Biomarker, VNN1