M.A. Thesis:

Investigating the possibility of producing hybrids of oyster mushroom (Pleurotus eryngii)

Abstract(include summary, goals, executive ways and results): Today, population growth and limited arable lands has led to an increasing demand for food every day. Therefore, as a good source of protein, edible mushrooms are of great importance in this regard.King oyster mushroom (Pleurotus eryngii) is of Basidiomycota fungi, protease families and plurotos kind. In addition to being edible and having a tender texture, this mushroom has medicinal and industrial uses as well. In this regard, some of the activities of the mushroom include stimulation and strengthening of the immune system, antioxidant properties, cancer control (preventing the growth of malignant tumors) and blood dilution, which leads to lowered cholesterol and blood fat improving the cardiovascular health of individuals. Provision of high-performance hybrid or modification with specific purposes are required to benefit from the mentioned properties of oyster mushrooms. In this study, spore extraction was directly performed, followed by culturing the single-spore isolates in potato dextrose agar (PDA) medium along with CYM nutrition. After 7-10 days, single-spores or primary mycelium grew, genomic DNA of which was extracted from mycelium tissue. In addition, polymerase chain reaction-based SSR primers were applied to distinguish monokaryon from daicarrion. Detected single-spores were selected as parents and participated in crosses. With respect to the incompatibility of two loci in the mushroom, molecular and phenotypic evaluation results and the ratio of number of successful crosses to the number of unsuccessful crosses, it was demonstrated that hybrid preparation in the assessment and selection of parents with SSR primers and single-spore method would be successful and replicable.

Keywords: Keywords : King oyster Mushroom (Pleurotus eryngii), Single-spore Selection, SSR Primer