M.A. Thesis:

: Screening and identification of organic solvents resistant bacteria to amylase enzyme production and metal nanoparticles biosynthesis investigation

The use of enzymes in organic solvents due to the reduced amount of water, reduce unwanted side products and On the other hand, thermodynamic equilibrium proceeds for products synthesis and goes up the enzymatic yield. The organic solvent by the separation of water surrounding the active site caused reduction of enzymatic activity. Achieving the protease resistant to organic solvents is a necessity for the industry. ?-Amylases of the most industrial enzymes and play a important role in modern biotechnology. Although several sources capable of producing ?-amylases. Generally, enzymes derived from microbial sources, especially from bacteria and Bacillus, have various industrial applications and more studied. In this study, The bacterial strains resistant to organic solvents has the ability to produce extracellular amylase. sequencing studies indicate high similarity of ASM-M1 strain to Bacillus subtilis strain. The optimum temperature and pH of the enzyme to 65 ° C and 4/7, respectively. SDS-PAGE electrophoresis revealed a bond with molecular weight of 50 kDa. The Km and Vmax of enzymes was 0.003 mM and 0.002 U / mg, respectively. The activity of this enzyme in the presence of organic solvents such as butanol different percentages, methanol, propanol, n-hexane, ethyl acetate was investigated. The most activities represented in the presence of methanol.

Keywords: Organic solvent resistant bacteria, -amylase, Sequencing, Purification.