M.A. Thesis:

kozak sequence effect on coagulation factor9 gene expression in insect drosophila s2 cells

Lack or deficiency of factor IX is known as a major factor in Hemophilia B and recombinant production of this factor is very important. There are different ways for expression increasing of recombinant proteins in expressions systems. Using kozak sequence as a regulatory sequence can be a way of expression increasing. In fact existence of this sequence before the start codon result to increasing efficiency of translation. In this study pMT-K-FIX express construction with Drosophila Kozak sequence was built in beginning of cDNA human factor and transfected to S2 Drosophila cells. Expression and activity amount of recombinant factor IX with Kozak sequence and normal factor IX was considered in different times by ELISA and coagulation. Results showed concentration and activity of factor IX with Kozak sequence was more than the normal factor IX in Drosophila S2 cell lines.

Keywords: Hemophilia B, factor IX, Kozak sequence, S2 cell line