M.A. Thesis:

The secretion efficiency of recombinant factor ۹ in hFIX producing S۲ cells

Secretion of proteins to outside of cells is the one of the ways to better purification of recombinant proteins in expression systems. Different expression systems may affect on secretion capability of recombinant protein with their different secretion mechanism or cell capability. So, in this research, we have compared the capability of secretion of a human recombinant protein such as factor 9 in drosophila system with a mammalian HEK system. The results have shown that the S2 cells have better capability for expression of recombinant factor 9 but theirs secretion efficiency is weaker

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