M.A. Thesis:

Expression of Human Clotting Factor IX in stable S2-hFIX cells By Real Time PCR

S2 cells expression system as a new expression system for production of many human proteins have been used. Ability of these cells for expression recombinant factor IX as a human protein is confirmed. Although expression of factor IX has been investigated at the protein level but expression at the transcriptional level has not been evaluated. So in this study, a mount of factor IX RNA by quantitative PCR was evaluated. The results of expression at the RNA level showed that the S2 cells capable of producing a human recombinant protein and also revealed that expression on consecutive days increased.

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