M.A. Thesis:

Comparison of recombinant FIX expression in stable CHO-FIX cells in small and large scale

The use of mammalian expression system for the production of recombinant proteins is increasing and in the meantime the most dominant CHO cells and the most common cells for production of recombinant proteins is. Due to the extensive technology and expression of differing views on the use of temporary or stable expression of recombinant proteins, stable expression so comparing and selecting the optimal method to generate invoices temporary CHO cells 9 in the could be useful. In this study, expression of 9 in laboratory or pilot scale and technology on a pilot scale transient expression were examined for expression of Factor 9. The results showed that transient expression in pilot mode 6 times and expressed in steady state is about 3 times higher than stated on the laboratory scale. The transient expression of protein production technology in pilot mode and the first 3 days of steady state is similar to the usefulness of this method for pre-clinical studies and approved.

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