M.A. Thesis:

Evaluation of the presence of vacA and ureAB genes in Helicobacter pylori isolated from patients with gastric ulcer in Sabzevar

Helicobacter pylori is the leading cause of gastrointestinal diseases. More than half of the adult population in developed countries and 90% of people in developing countries are infected with Helicobacter pylori. Helicobacter pylori infection may be related to environmental and genetic factors. The aim of this study was to evaluate the frequency of Helicobacter pylori and vacA and ureA genes in isolated samples from patients with gastric ulcer. Materials & Methods: This descriptive cross-sectional study was performed on 95 samples of patients with gastric ulcer in Sabzevar. After DNA extraction using specific primers of the two genes and PCR test was performed frequently; In this study, urease test was performed on the samples. Results: Out of 95 samples of patients with gastric ulcer, 45.3% of the samples were infected with Helicobacter pylori. The presence of ureA gene was reported to be 28.4%. The frequency of vacA gene was 34.7%. In this study, 22.1% of the urease test was positive. According to the Chi-square test and analysis of the results, no significant relationship was observed between Helicobacter pylori infection and the presence of genes and gender. But there was a significant relationship between the presence of vacA and ureA genes and Helicobacter pylori infection.

Keywords: Helicobacter pylori, PCR, vacA gene, ureA gene, gastric ulcer, Sabzevar city