Thesis:

Investigating the effect of rosemary extract (Rosmarinus officinalis) and its nanoparticle on the collagen synthesis process in wound healing in diabetic mice model

Diabetes is the seventh leading cause of death globally, and one of its chronic complications is impaired wound healing in diabetic patients, which may ultimately result in amputation. The present study aimed to enhance wound healing and evaluate collagen synthesis through the development of an ointment incorporating herbal formulations and silver nanoparticles synthesized using rosemary extract via a green synthesis approach with an eucerin base. The morphology of the synthesized nanoparticles was characterized using scanning electron microscopy (SEM), confirming optimal shape and distribution under the selected conditions. In the in vivo study, male Syrian mice were rendered diabetic by streptozotocin administration and compared with a control group. Following anesthesia and the creation of dorsal wounds, the treatment groups received daily applications of the formulations for 21 days. On day 7, biopsies were collected, and collagen content was quantified using a hydroxyproline assay kit. Statistical analysis revealed that the group treated with the rosemary extract–silver nanoparticle formulation exhibited a significantly higher wound closure rate compared to the other treatment groups and the control. .

Keywords: Additionally, collagen levels in this group were significantly elevated relative to the other groups