Thesis:

Investigating the epidemiology of human tuberculosis related to bovine tuberculosis in Sabzevar city by PCR method

Tuberculosis has long been one of the most important diseases that threaten human life. In order to identify the source of infection, the method of disease transmission and epidemiology, during these years, tubercular cows were researched and studied in slaughterhouses across the country. In this way, in this study, Mycobacterium bovis isolated from slaughtered cows was investigated molecularly to determine the relevance of bovine tuberculosis bacillus in human infections. This descriptive-cross-sectional study was conducted in a period of one year (from April 1401 to 1402) from 150 cows over two years old suspected of bovine tuberculosis, tissue samples (lymph nodes and blood) and from people working in cattle farms. who were positive for bovine tuberculosis in the PCR test, sputum and blood samples were taken. All the samples were checked for Mycobacterium bovis by Zil-Nelson staining, culture in specific medium and PCR. The results of microbial culture tests and confirmatory tests revealed that the highest frequency of contamination of livestock with Mycobacterium bovis is in samples related to lymph nodes; In the samples tested by PCR method, the frequency of infection was determined in the lymph nodes of eight cases and the blood of four cases. In this research, the cows that were PCR positive were referred to the respective cattle farms and 64 blood and sputum samples were taken from the people working there.

Keywords: Human tuberculosis, Bovine tuberculosis, Mycobacterium bovis, PCR