Thesis:

Investigation of Brucella bacterial contamination in some dairy products of Jajarm city by PCR method

Sampling was done from 12 samples of cow's milk, industrial cattle farms, 6 samples of cream and 5 samples of cheese from dairies in Jajarm city. First, milk ring test was performed and then DNA was directly extracted from milk, cream and cheese samples and finally PCR test was done using B4 and B5 primers. The results of this research showed that despite the negative results of the milk ring test in Brucella detection, the PCR technique was able to identify 2 cases out of 23 studied samples. Out of 12 cow's milk samples suspected of brucellosis, 1 case and out of 5 dairy cheese samples was infected with Brucella bacteria. This research showed that the PCR test is an accurate and efficient method for the precise search and specific detection of Brucella. Therefore, it is expected that the process of production and pasteurization of these dairy products will be revised and a serious monitoring will be done. In order to determine the identity of Brucella, in addition to the culture method, it is suggested to use the polymerase chain reaction and the use of specific primers that are able to detect all the biovars of Brucella abortus and Brucella milli-tensis

Keywords: Jajarm city, Brucella, milk ring test, polymerase chain reaction,PCR