M.A. Thesis:

bactrrial flora examination based on age and weight

Oral normal flora plays an important role in oral and dental health. In addition to the mouth and teeth, other parts of the body can also be affected by the natural flora of the mouth. Oral flora can also cause systemic infections, digestive diseases, and so on. The natural flora of the mouth can be bacteria or fungi or other germs. Of course, some of these microorganisms have beneficial effects for their host body, and most of them do not have a well-known function. The abundance of food, dead epithelial cells, and salivation make the oral cavity a favorable environment for the growth of bacteria. Most of these bacteria are innocuous in normal conditions. However, some of them can cause dental caries or gum disease in certain conditions. Streptococcus mutans are the most important bacteria in tooth decay and porphyromonase gingivillis is the most important anaerobic bacteria in gum disease. The aim of this study was to evaluate bacterial flora based on age and weight. Also, as a subjective goal, the difference between oral flora differences between diabetic patients and healthy subjects was investigated. In this regard, 130 patients were referred to the treatment centers of bacterial samples. Specimens were cultivated in differential and specific environments. Then bacterial samples isolated by PCR assay for presence of mutant family genes were investigated. A total of 130 people were male (%). The mean age of the participants in this study was 46.7. Of those, the diabetic population was 36.1%. The results of bacterial culture indicated the presence of bacterial strains of Streptococcus mutans. The results did not show any significant difference between the oral flora of healthy subjects and diabetics. In addition, the presence of mut I, II, II genes was confirmed in Stertococcus mutans. In general, it can be concluded that there is no significant relationship between the natural flora species of the mouths of people with their age and gender. Also, diabetes mellitus does not affect the natural flora of the mouth. Also, according to the results of the prevalence of mutant gene in samples of people living in Mashhad in Streptococcus mutant species, 92.2%

Keywords: S. mutans P. gingivalis S. sanguinis)