M.A. Thesis:

The study of long non coding RNA (Miat) expression in Stroke patient

Background AND Purpose: Stroke is one of the most common diseases in the world, and the rapid and accurate diagnosis of molecular factors involved in this disease is very important. Several studies have shown that lncRNAs act not only as mediators between DNA and proteins, but also play an important role in cellular function. LncRNAs can regulate gene expression in a variety of ways, such as chromosomal changes, transcription, and post-transcription processing. In addition, lncRNAs disorder is particularly relevant to many human diseases, including cerebrovascular disease, heart disease, and cancer. In this study we investigated lncRNA miat activity in blood samples of patients with stroke. Materials and Methods: Total RNA from blood samples of people with stroke and healthy people, extraction and quantity and quality were determined by using nanodrop and gel electrophoresis. Then, cDNA synthesis was performed. Conditions were determined by optimum PCR technique and the desired band after electrophoresis with gel duct Observation, and finally evaluated the gene expression by PCR real time technique. Results: According to the results of this study, the expression of lncRNA miat in blood samples of patients was 2.6 times higher than healthy ones. Therefore, with further research, this gene could be used for diagnostic purposes and targeted therapies to help these patients.

Keywords: lncRNA Miat, real time PCR, Brain Stroke