M.A. Thesis:

Comparative study of antibacterial and nanoparticle biosynthesis of saffron (Crocus sativus) and several medical plants and evaluation of its influence on alpha-amylase inhibition

Plants have various applications in human life including food and pharmaceutical applications. In recent years there have been increasing interest in pharmaceutical applications of plants. To evaluate the antimicrobial properties of saffron (Croucus sativus L.), Salvia leriifolia, Rheum palmatum L., Eryngium billardiera, Silybum marianum, Arctium lappa and Vaccinium avctostaphylos against bacterial pathogens of Staphylococcus aureus, Echerishia coli, Pseudomonas aeruginosa, Klebsiella Pneumonia, n-hexane and alcoholic extracts of these plants were used by microbroth dilution method. Plant extracts were prepared by maceration method and methanol and n- hexane solvents. The study showed that highest inhibitory effects is for Silybum marianum at the concentration of 15 milligrams per milliliter and lowest inhibitory effect is for saffron petals with 180 milligrams per milliliter. Bernfeld method were used to measure inhibition of the alpha-amylase enzyme. N-hexane and alcoholic extracts of mentioned plants have been used to inhibit the alpha-amylase, best and highest inhibitory effect were recorded for methanol extracts of Eryngium billardiera and saffron stigma. Laynurberg curve showed that plants’ extracts inhibit the enzyme competitively. Biosynthesis method were used for nanoparticle production. The herbal extracts were used as reductive agents and absorption spectrum in range of 400 to 550 nm in UV-Visible spectroscopy showed that nanoparticle synthesis was performed by plant extracts.

Keywords: Saffron, inhibitior, alpha amylase, Bernfeld, nanoparticles