M.A. Thesis:

Investigation of bacterial contamination in trout pools by pcr method

The rapid identification of bacterial pathogens in salmon is of particular importance. Therefore, PCR method was used to detect bacterial infection caused by Yersinia rachria bacteria from the saline fish pool of salmon farms. The present study was conducted in a descriptive cross sectional study for six months during summer and autumn of 1397 in 10 rainbow trout farms in Sabzevar.At First,sampling was done from the pool water and then the suspended material was deposited in a sample by centrifugation and then microbial culture and warm bacterial staining were performed and PCR was used to diagnose. Out of 10 farms, 4 farms had symptoms of suspected Yersiniosis, of which 2 positive microbial cultures and 2 other non-growth-producing fields were declared.In 6 other farms that did not have symptoms, bacteria were not isolated after microbial culture. In the other 2 farms that were cultured with a positivi microbial method, the PCR method was not positive and the target band was not created. According to the results of the study, farms in Sabzevar county have a lower infection rate than bacteria Yersinia rachria...

Keywords: Yersinia racari, trout, pathogen, PCR, pool water