M.A. Thesis:

The effect of simultaneous mutations in amino acids ۱۳ and ۱۴ of propeptide on the expression of human factor IX

Hemophilia is one of the most difficult and most costly human diseases, and the person suffering from it is spontaneously, or as a result of the smallest blow or injury, with bleeding, which is accompanied by severe bruises, semen, hematoma and severe pain. These patients The incidence of hemorrhage, if not rapidly replaced in special treatment centers, is treated with special care and injected with blood products, has wide and painful hematomas, disabilities, extensive joint abnormalities, amputations, and even death.Hemophilia B is a condition in which the coagulation factor IX, which is dependent on vitamin K, is not produced at all, or is not well carbohydrates by the gamma carboxylase enzyme. The incidence of vitamin K protein-related protease in gamma carboxylase indicates the specific amino acids in the protein sequence. The gamma-carboxylase enzyme tends to prothrombin. In this study, we tried to construct a pMT-FX-M expression construct, having a mutated factor IX mutated cDNA of 13- and 14-bis, based on prothrombin, which resulted in production, activity and gamma-carbosilation The mutated Factor XI grew to its original state during the same conditions and times. This study was performed by ELISA and coagulation tests.

Keywords: Factor IX-Gamma Carboxylase-Propoptide – Prothrombin