M.A. Thesis:

detection of Gense Involved in Nivalenol and deoxynivalenol Mycotoxins of Fusarium spp.Isolated from Maize and soyben meal used in laying poulty of khorasan razavi province

Abestrac Contamination to fungi that produce mycotoxins is one of common problems of food that resulted in a broad spectrum of clinical effects on humans and animals.In this study, according to importance of corn and soya meal as the main cereal for animal feed, the fungal flora of soya meal and corn sampels used in 16 laying poulty of Khorasan Razavi province, Iran, during the first tri month of the year 1395 were investigated. Following humidity measurement, according to the national standard of 997; YGC and PDA culture medium were used for the isolation and enumeration of fungal flora. After purification of isolated strains, the isolates wee identified macroscopically and microscopically and if necessary culture slides prepared. Also, Rapid detection of fusarium contamination and identification of Fusarium strains that have genes produced nivalenol and deoxynivalenol mycotoxins were done with PCR method. According to results of PCR reactions with fusarium specific primers, of all 100 fungal DNA suspected as fusarium, 78 isolates confirmed. But the results of the PCR reactions with fusarium specific primers, of all 100 fungal DNA suspected as fusarium, 78 insolates confirmed. But the results of the PCR reaction with specific primers F.culmorum showed no isolate. In addition, 5/5% of all isolatesremained unknown. Results of molecular identification demonstrated a 11/5% presence of species that produce deoxy nivalenol and nivalenol, so that 4 of fusarium isolate that produce deoxy nivalenol blonesd to F.graminearum. moreover, number of 3 isolates have identifind as F.graminearum.

Keywords: Deoxynivalenol, Nivalenol, Mycotoxin, Fusarium graminearum