M.A. Thesis:

Investigation effects of culture media and growth regulators concentration on proliferation and rooting of Rosa damascena mill cv "kashan ۲"

Rosa damascena Mill is one of important medical and economical plants in the world and in Iran. The plant importance is in rose water and essence production from its petals, that has many usages in medical, ornamental hygienic industries. It also applies in food industry. To determining the best hormone compound and medium to Prolifcation and rooting rosa damascena tiny-samples was done in factorial form and completely with 5 times repetition in Bojnourd laboratory of plant-fiber cultivation of Islamic Azad University. In the first stage regeneration test of branches had been studied in reaction to their hormone compound and medium compound. The study factors included different levels of growth regulators (NAA,BAP) in 2 medium types (MS,WPM).So it had been used of one-node pieces of cultured plant stems in greenhouse for tiny-samples. Results showed that there had a meaningful difference between applied mediums and different growth regulator density in relation with properties of branches regeneratio. Maximum percent of branch regeneration, branch numbers in tiny-samples and average branch length were observed respectively in density BAP 2 mg/l without hormone NAA, BAP 2 mg/l with NAA 0.5 mg/l in WPM medium and BAP 1.5 mg/l with NAA 0.5 mg/l in WPM medium. Timar with 0 mg/l BAP in MS medium had the least branch regeneration percent, branch number in tiny-sample and branch length.Growth regulator application NAA in medium had caused to decrease in branch regeneration percent, yet NAA application had caused to meaningful increase in number and length of branch in mediums containing BAP 2 mg/l. In the second stage of test, it had been evaluated the reaction of mediums with different densities of IBA regulator for branches ramify. Ramify results showed that the best function had occured in MS medium containing IBA 1 mg/l

Keywords: Rosa damascena, In vitro culture, Cytokinin, Auxin, Regeneration