M.A. Thesis:

survey of antibacterial potential of Lucilia sericata larval secretions on E.coli

Wound infection is a universal Problem to chronic wounds treatment. This problem is compounded by the widespread occurrence of ‘antibiotic resistant bacteria. Larva of the green bottle fly Lucilia sericata are increasingly widely used as a fast and effective means of treating wounds, particularly chronic wounds where conventional treatments have failed. In recent years, several reports have described the presence of fractions with antibacterial activity, in the excreta/secreta(ES) of the larvae. However, the molecular mechanisms involved in antibacterial activity by ES are not very well understood. The study of the changes in gene expression at the transcriptional level may yield insights into understanding the molecular mechanisms that have antibacterial effect and may provide new and more efficient treatment for chronic wounds. In this study The antibacterial effects of larvae ES against multiantibiotic resistant E.coli and also MREB and RPOC genes expression change of ES treatment E.coli investigated. The result showed that infected larvae ES had bacteriostatic activity agains E.coli. the expression of both studied genes increased in the lava ES treatment E.coli. in the other words, larvae ES control and inhibition of bacterial growth together with remain bacterial vital activity. due to this results; larvae ES in addition to the therapeutic behavior have the potentional to be used in biotechnology

Keywords: Lucilia sericata, MREB, RPOC