M.A. Thesis:

Staphylococcus aureus isolated from the host node Cocos available in food stores Sabzevar PCR method.

Staphylococcus aureus is a pathogen that, under favorable conditions, can cause food poisoning in humans by growing in food and producing enterotoxin. Only some strains of Staphylococcus aureus can produce enterotoxin and cause food poisoning. By using DNA replication methods, the presence of enterotoxogenic strains of Staphylococcus aureus can be identified based on a specific gene sequence. The aim of this study was to identify Staphylococcus aureus strains capable of producing Enterotoxin A. In this research, samples of hamburgers taken from Sabzevar city shops were cultivated on gram positive and gram negative culture media. Samples that were grown on a positive gram-positive culture were approved Diagnosis was cultured on Mannitol Salt Agar medium. DNA positive specimens were extracted by Sina Clone Extraction Kit and isolated with specific primer for Entrotoxin A for each of the samples according to the PCR protocol. Among the 90 hamburgers, only 2 samples They contain the Enterotoxin A gene.

Keywords: Staphylococcus aureus - Entrotoxin A- PCR