M.A. Thesis:

Evaluation of salmonalla contamination in eggs native city sheshtemad

Salmonella The gram-negative bacilli belong to the Enterobacteriaceae family. Salmonella contamination has been reported in animals such as cattle, horses, pigs, dogs and cats, and sheep and poultry, which causes mild gastroenteritis in a deadly toxoid in humans. Eggs are one of the most complete natural foods. Salmonella is a common contamination in eggs. This bacterium reveals its symptoms within 6 to 48 hours. Considering the importance of eggs in transferring salmonellosis and the high consumption of local and non-industrial eggs in the region, it was also necessary to find out the level of salmonella infection in Sheshtemad city. In this research, we examined the contamination of eggs. After sterilizing its crust with 70% alcohol, its contents were cultured on standard media such as Salmonella chicla agar, McCanky Agar and ... and tested by catalase and urease test. Subsequently, PCR testing was used to determine Salmonella specificity. Of the 60 eggs examined, 2 eggs (3. 3%) were contaminated with Salmonella typhimurium and Salmonella enteritidis. This infection was present only in the egg shell and was not confirmed in the contents of egg infection by PCR. Salmonella Typhimurium isolated from egg shells probably originates from indigenous chickens, infected beds, humans or other environmental agents.

Keywords: Salmonella typhimurium, Salmonella enteritidis, eggs, PCR