M.A. Thesis:

The effect of plant growth regulators on shoot regeneration and in vitro rooting of Damask rose (Rosa damascena Mill.)

To evaluate the effect plant growth regulators on shoot regeneration and in vitro rooting of Damask rose, two experiments were done in biotechnology laboratory of Islamic Azad University of Sabzevar. At first, the effect of BAP concentrations (0, 0.5, 1, 1.5, 2 and 2.5 mg/l) on shoot regeneration were studied in a completely randomized design with four replications. After selection of the best concentration of BAP for in vitro regeneration of shoots, the effect of IBA (0.5, 1 and 1.5 mg/l) and NAA (0, 0.5 and 1 mg/l) concentraions on rooting of shoots were investigated. The experiment was carried out factorial arrangement based on completely randomized design with three replications. The results showed that BAP concentration had significant effects on shoot regeneration percent, shoot number per explant, shoot length and leaf numer per shoot. The regeneration percent of shoots increased with increasing BAP concentration to 2 mg/l and then declined significantly. The increasing BAP concentration to 1.5 mg/l produced the highest number of shoots per explant. There were no significant difference between 1.5, 2 and 2.5 mg/l BAP concentrations for number of shoots per explant. The maximum length of shoots was obtained at 1.5 mg/l BAP concentration and increasing BAP concentration significantly decreased it. Application of 0.5 mg/l BAP produced the highest number of leaf per shoot. Higher concentrations of BAP decreased number of leaf. The effect of IBA and NAA concentrations were significant on all rooting characteristics. There was no rooting in a concentration of 0.5 mg/l IBA. In culture media containing 1 mg/l IBA, only the use of 1 mg/l NAA was induced rooting in shoots. The highest percent of rooting and number of roots per shoot were observed on calture medium containing 1.5 mg/l IBA and 0.5 mg/l NAA. However, medium containing 1.5 mg/l IBA and 1 mg/l NAA had the highest root length. According to the results, the use of BAP at a concentration of 2 mg/l in MS culture medium is desired for shoot regeneration in Damask rose. The MS medium containing 1.5 mg/l IBA and 0.5 mg/l NAA for in vitro rooting of Damask rose is recommended.

Keywords: Auxin, shoot regeneration, rooting, cytokinin, Damask rose