M.A. Thesis:

anti-bacterial effects of methanolic and ethanolic extract of fruit Solanum Nigeruml.against food borne pathogenes

Yellow nightshade plat, herbaceous plant, annual, short, long it is less than half a meter.Bacteria like Escherichia coli, Salmonenlla typhymurium, staphylococcus aureus, and Lysteria innocua are known as human pathogensand food contaminations. In this study we investigate the effect of Yellow nightshade fruit Mthanolic and Ethanolic on the bacteria we mentioned above.After collecting the fruit this plant in late summer the Metanolic and Ethanolic extracts of this plant can be produced. For Metanolic and Ethanolic extracts there are different concentration rates ( like 320، 160، 80، 40، 20، 10، 5، 2/5، 1/25، 0/62 mg/ml) which has been produced by microdilution Broth method in BHI medium and culteration in Mueller Hinton Agar medium. The minimum inhibitory concentration(MIC)and minimum bactericidal concentratio (MBC)have been determind with tow metods of visual monitoring and optical density (OD) with use of Elisa Reader.The resalt have shown that Ethanolic extract of Yellow nightshade fruit whit minimum inhibitory concentration for E. coli and L. innocua 320mg / ml for S. typhymurium 160 mg / ml and Staphylococcus aureus 80mg / ml and the minimum bactericidal concentration (MBC) for bacteria Staphylococcus aureus 80mg / ml for Salmonella 160mg / ml for Listeria innocua 320mg / ml and for there was no E. coli MBC. that Mthanolic extract of Yellow nightshade fruit whit minimum inhibitory concentration for the bacteria E. coli, Staphylococcus aureus and S. typhymurium 80 mg / ml and bacteria Listeria innocua 160 mg / ml is. The minimum bactericidal concentration (MBC) for Staphylococcus aureus and Listeria innocua 160mg / ml for Salmonella 320mg / ml and for E. coli MBC did not exist.

Keywords: antibacterial effect, yellow nightshade, methanol extract, methanol extract