M.A. Thesis:

Media optimization for α-amylase production by Bacillus amyloliquefaciens

Employing biotechnology in industry is very common in the current age. One of the industrial functions of biotechnology is utilizing microorganisms in the production of enzymes. Using bacterial and fungal sources in the commercial production of enzymes has several advantages. In addition to being cost-effective, they are also easily available and manipulated. The bacillus bacteria is an important genus in the industrial production of enzymes. One of the most useful enzymes employed in different fields like food production, medicines, textile industry etc. is alpha-amylase. Alpha-amylase breaks the adjacent links of sugar in starch, turning it into units of glucose, maltose and maltotriose. The bacillus amyloliquefaciens is an important genus in the industrial production of heat-resistant alpha-amylase enzyme, and with altering some environmental factors, it would be possible to improve the enzyme’s production using this strain. Chief among the various aims of improving the environmental factors is a largescale production of the enzyme with high efficiency and minimum expenditure. In this study, attempts have been made to improve the production of this alpha-amylase enzyme for industrial purposes through altering the environmental factors. The study revealed that the highest amount of alpha-amylase synthesis was executed by bacillus amyloliquefaciens bacteria in a growth medium with pH=6 and in 48 hours following the start of the reaction, with the amount of starch, yeast extract and calcium chloride being 21.55 gr/lit, 2 gr/lit and 2 gr/lit, respectively. Providing said environment thus helps the bacillus amyloliquefaciens bacteria to play the highest function in the alpha-amylase synthesis.

Keywords: Optimization, alpha amylase, Bacillus amyloliquefaciens