M.A. Thesis:

detection and determination of kanamaycin based on aptamer٫picogreen and gold nanoparticles

The biosensor is used extensively in identifying chemicals. These methods smarter, faster and often cheaper than laboratory methods to identify materials offer. Biosensor various mechanisms they use. One of these types of biosensors, Opta sensors are used to identify aptamers that the materials they use. Aptamers are single-stranded oligonucleotides and small three-dimensional structures which are specific to the target biomolecules such as amino acids, drugs, proteins and other molecules with high affinity and specificity to be connected with the help of certain identifiers, such as color or fluorescent materials the materials are detected. Kanamycin, an important subset of aminoglycoside antibiotics, which are widely used for the treatment of serious infections caused by Gram-positive and Gram-negative bacteria by interfering with protein synthesis is used. In this study, we, the Pico green color that is a signal probe as fluorescent markers, used and new design for kanamycin Aptasnsvr fluorescence, fluorescence Aptasnsvr to the conclusion that this is a very sensitive and specific for kanamycin.

Keywords: biosensor,aptamer,kanamycin,picogreen