M.A. Thesis:

Tracking staphylococcus aureus Enterotoxin Aand B genes in butcherys of Sabzevar by PCR

Abstract This study aims at separating Staphylococcus aureus from the meat supplied in Sabzevar's butcher's shops , detecting Staphylococcic A and B Enterotoxins genes through PCR method , and checking its dangers on society. 12 types of Staphylococcus were separated from 42 types of prepared meat Swab. By doing Gram coloring , Catalase test , DNase test , Manitol , and culturing in salt phenol red agar and Macconkey agar , 12 types ( 28.57%) of 42 microbial types were identified as Staphylococ that 8 types were Staphylococcus aureus and 4 types were from other types of Staphylococs. DNA extraction was done by boiling in presence of KOH and Staphylococcic A and B Entrotoxin genes were tracked by PCR method. According to the obtained results and due to the importance of Staphylococcic Enterotoxins in poisoning foods and endangering the community health , the suggestion is to get the home meat from hygienic and licensed butcher's shops and hypermarkets . In addition, meat is supplied , carried to the butcher's shops and meat sellers , and kept in cold temperature according to the health rules and protocols. However, these centers producers and workers should be necessarily informed with training courses and the manpower who works in the meat production and distribution cycle should be provided with the sufficient , useful information about the threatening dangers to the community health.

Keywords: Keywords: Staphylococcus aureus , PCR , A and B Enterotoxin genes , DNA extraction