M.A. Thesis:

Comparison in preparation of stable clones HEK-FIX with S2-FIX

Large-scale production of recombinant proteins requires the preparation of stable cell clones. However, preparation of stable clones for different cell lines is different. In this study, optimum conditions for the preparation of stable clones producing clotting factor 9 in two S2 insect cell line mammalian HEK cells was evaluated. In addition, expression and activity of factor 9 from stable cell lines were compared. The results showed that the preparation of stable clones in S2 cells is much shorter than the HEK cells as well as methods of using mitomycin is effective in the preparation of S2 stable clones.

Keywords: pcr-Factor9- mitomaycin-