M.A. Thesis:

Study the possibility of vegetative propagation of pistachio cultivar Badami-sefid through tissue culture

In order to evaluate the effect of hormonal composition of culture medium on micropropagation of UCB-1 pistachio cultivar, two factorial experiments in the form of completely randomized design with four replications were carried out at Biotechnology Laboratory of Islamic Azad University, Sabzevar Branch. In the first stage of experiment, shoot regeneration response to hormonal composition of culture medium was studied. Experimental factors include BAP concentrations at four levels (0.5, 1, 1.5 and 2 mg/l) and IBA concentration at two levels (0 and 0.1 mg/l). The results showed that BAP concentration had significant effect on shoot regeneration characteristics. The highest shoot regeneration percentage, shoot number per explant and shoot length were observed in 1.5, 2 and 1 mg/l BAP concentration, respectively. The treatment containing 0.5 mg/l BAP had the minimum shoot regeneration percentage, shoot number per explant and shoot length. Application of 0.1 mg/l IBA in the culture medium decreased shoot regeneration percentage, especially in the medium containing 1.5 and 2 mg/l BAP. However, application of 0.1 mg/l IBA significantly increased shoot number and length in the medium containing 2 mg/l BAP. After selection of the best hormonal composition of culture medium for shoot regeneration, the effect of different concentration of IBA (0.5, 1 and 2 mg/l) and NAA (0,0.1 and 0.5 mg/l) on in vitro rooting of shoots were investigated. 45 days after the transfer of UCB-1 shoots to rooting media, no rooting was observed in shoots.

Keywords: Auxin, pistachio, UCB-1, shoot regeneration, rooting, cytokinin