M.A. Thesis:

the effect of carbon nanotybes on optimizarion of callus induction on saffron

In order to optimization of saffron Callogenesis with different concentrations of hormone in terms of carbon nanotubes, a Response Surface Methodology experiment was carried out in Islamic Azad University of Sabzevar in 2013. In this study, different concentrations of hormones 2-4-D (0.5-0.2 mg/l) and BAP (1.5-6.5 mg/l) and different combinations multi-walled carbon nanotubes (30-70 mg/l) were tested for Measuring callus induction. The results showed that Quadratic function was close-fitting for the number of callus, callus diameter, callus weight and percentage of Callogenesis. The highest callus production was observed in application of 0.5 mm of 2-4-D and concentrations of 25 mg of multi-walled carbon nanotubes. In lower concentrations of 2-4-D, increase the amount of multi-walled carbon nanotubes decreased callus induction percentage but in higher concentrations of 2-4-D, increase the amount of multi-walled carbon nanotubes increased callus induction percentage. Variations in the number, weight and callus diameter was nearly similar in application of the different concentrations 2-4-D and BAP hormones. So that in low and high concentrations of BAP, with increase the amount of BAP, increased Measured factors of the this study. Number, weight and callus diameter increased with accumulative concentration of carbon nanotubes with BAP. Generally optimization results showed that the highest callus diameter, weight and callus induction percentage (18.81mm, 0.26mg and 91.92%) in terms of consumption 0.5 mg 2-4-D, 1.5 mg BAP and 28.94 mg of multi-walled carbon nanotubes was observed.

Keywords: saffron, Optimization, Response Surface, multi-walled carbon nanotubes