M.A. Thesis:

Evaluation of affecting factors on expression of coagulation factor IX in S۲ system

HaemophiliaBis an of X- linked recessive blood clotting disorders caused by a mutation of the factor IX gene, leading to a deficiency of factor IX. It is the second-most common form of haemophilia, rarer than haemophilia A. De?ciency in coagulation factor IX is the most common cause of hemophilia B, which occurs in 1/30,000 males. Treatment or prevention of bleeding episodes in haemophilia B patients is based on the injection of coagulation factor IX (replacement therapy) concentrated from pooled human plasma or from recombinant preparations. We also assay the effect of vitamin K concentration and amount of DNA (recombinant vector containing hFIX gene) used for transfection on the expression of rhFIX in Drosophila S2 cell line. We showed that the concentration of vitamin K and amount of DNA could affect the expression of rhFIX in Drosophila S2 cell line.

Keywords: Haemophilia B- factor IX- vitamin K- rhFIX